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Imunologia e Infecção
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Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

DOI:
10.3791/2820-v

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11:29 min

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December 03, 2011

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, , , , ,
1Laboratory of Water and Food Viral Pollution, Department of Microbiology, Faculty of Biology,University of Barcelona

Capítulos

  • 00:05Título
  • 00:11Introduction
  • 01:56Collection of Water Samples
  • 02:45Viral Concentration by Organic Flocculation
  • 06:10Nucleic Acid Extraction and Quantification of Viruses
  • 10:46Conclusion

Summary

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The study describes a cost-effective method for the identification of the source of fecal/urine contamination or contamination by nitrates in water using qPCR for the specific quantification of human/porcine/bovine DNA viruses, adenoviruses and polyomaviruses, proposed as MST tools.

Tags

Microbial Source TrackingCost-effective MethodSpecific Human VirusesSpecific Animal VirusesViral IndicatorsFecal ContaminationWater QualityMolecular AssaysAdenovirusesPolyomavirusesQuantitative PCR (qPCR)Porcine AdenovirusesBovine PolyomavirusesWater Sample AnalysisNitrates

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