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10:47 min
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July 01, 2011
DOI:
1Department of Medicine, David Geffen School of Medicine,University of California, Los Angeles, 2Research service, 151,Veterans Affairs Greater Los Angeles Healthcare System, 3Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics,University of California Los Angeles (UCLA), 4Division of Infectious Diseases, 111F,Veterans Affairs Greater Los Angeles Health Care System
Summary
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一种有效的方法来评估钩端螺旋体蛋白质表面暴露描述。该方法是专门设计，以避免破坏脆弱的钩体细胞的外膜。这种技术需要雇用几个阴性对照，以评估外膜的完整性和特异性抗体反应。
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Cite this Article
Pinne, M., Haake, D. Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins. J. Vis. Exp. (53), e2805, doi:10.3791/2805 (2011).
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