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量化传播肿瘤细胞的频率使用斑马鱼的同源有限稀释细胞移植
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish
DOI:

08:01 min

July 14, 2011

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Capítulos

  • 00:05Título
  • 01:40DNA Microinjection of Zebrafish Embryos
  • 02:47Screening for Primary T-ALL in Zebrafish Larvae
  • 03:25Isolation and Purification of Fluorescently-labeled Primary Leukemia Cells
  • 04:57Limiting Dilution Transplantation into Adult Zebrafish
  • 05:31Analysis to Determine: Leukemia-initiating Cell Frequency
  • 06:15Representative Results
  • 07:26Conclusion

Summary

Tadução automática

有限稀释细胞移植试验是用来确定传播肿瘤细胞的频率。本协议描述了一个产生,发展荧光标记的白血病和细节,如何限制稀释到成年斑马鱼的腹腔这些白血病细胞分离和移植的同源斑马鱼的方法。

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