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Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish
JoVE 杂志
生物学
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JoVE 杂志 生物学
Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish

Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish

18,885 Views

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08:01 min

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July 14, 2011

DOI:

10.3791/2790-v

DOI:
10.3791/2790-v

•

08:01 min
July 14, 2011

•

3 Views
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1Department of Molecular Pathology, Massachusetts General Hospital,Harvard Medical School, 2Department of Molecular Pathology, Massachusetts General Hospital Cancer Center,Harvard Stem Cell Institute

Tags

QuantifyingFrequencyTumor-propagating CellsLimiting Dilution Cell TransplantationSyngeneic ZebrafishSelf-renewing Cancer CellsTumor GrowthTumor-initiating CellsDestructionTumor ProgressionRelapsePatient PrognosisDetermining FrequencyTransplantation AssayRecipient AnimalsIncreasing DosesDevelop TumorsOriginal Tumor SampleLarge Number Of AnimalsLimiting Dilution ExperimentMiceCostlyZebrafish As A Cancer ModelGenetic ManipulationLarge Scale ExperimentsCancer Types Modeled In Zebrafish

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Blackburn, J. S., Liu, S., Langenau, D. M. Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish. J. Vis. Exp. (53), e2790, doi:10.3791/2790 (2011).

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