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유전과 Epigenetic 분석을위한 파라핀 임베디드 재료에서 DNA 추출
JoVE Journal
Biologia
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JoVE Journal Biologia
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
Please note that all translations are automatically generated. Click here for the English version.

유전과 Epigenetic 분석을위한 파라핀 임베디드 재료에서 DNA 추출

55,338 Views

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13:32 min

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March 26, 2011

DOI:

10.3791/2763-v

DOI:
10.3791/2763-v

•

13:32 min
March 26, 2011

•

28 Views
, , ,
1Department of Integrative Oncology,BC Cancer Research Centre, 2Interdisciplinary Oncology Program,University of British Columbia – UBC, 3Photography/Video Production, Multi-Media Services,BC Cancer Agency, 4Department of Pathology and Laboratory Medicine,University of British Columbia – UBC

Summary

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이 동영상은 포르말린 고정 파라핀 - - 임베디드 물질로부터 DNA 추출을위한 프로토콜을 보여줍니다. 이것은 조직 섹션 크실렌과 deparaffinized하는 멀티 일 절차, 에탄올과 rehydrated 이후 유전자의 특정 또는 게놈 차원의 분석을 위해 DNA를 정화하고 분리 proteinase K로 처리됩니다.

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Pikor, L. A., Enfield, K. S. S., Cameron, H., Lam, W. L. DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses. J. Vis. Exp. (49), e2763, doi:10.3791/2763 (2011).

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