/
/
Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
This content is Free Access.
Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
15,255 Views
•
08:38 min
•
May 25, 2011
DOI:
1Section on Synaptic Pharmacology/Laboratory for Integrative Neuroscience,National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism, 2Department of Electronics Engineering,Ewha Womans University, 3Section on Transmitter Signaling/Laboratory of Molecular Physiology,National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism
Summary
Automatically generated
This report demonstrates a technique for mechanical isolation of individual viable neurons retaining attached presynaptic boutons. Vibrodissociated neurons have the advantages of rapid production, excellent pharmacological control and improved space-clamp without influence from neighboring cells. This method can be used for imaging of synaptic elements and patch-clamp recording.
Explore More Videos
VibrodissociationNeuronsRodent Brain SlicesSynaptic TransmissionPresynaptic TerminalsMechanical DissociationIsolated NeuronExtracellular EnvironmentPostsynaptic Intracellular EnvironmentMicropipetteVibration-based TechniqueP1-P21 RodentLiberation Of Isolated NeuronsPrimary Neuronal CulturesEnzymatically Isolated NeuronsElectrophysiological StudiesImaging StudiesPharmacological ExperimentsRapid Drug ApplicationTotal Cell SuperfusionWhole-cell Recordings
Read Article
Cite this Article
Jun, S. B., Cuzon Carlson, V., Ikeda, S., Lovinger, D. Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals. J. Vis. Exp. (51), e2752, doi:10.3791/2752 (2011).
Copy