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在 C 在体内激光干切断线虫

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

In vivo Laser Axotomy in C. elegans

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在 C 在体内激光干切断线虫

DOI:

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09:18 min

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May 19, 2011

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Alexandra B. Byrne*¹, Tyson J. Edwards*¹, Marc Hammarlund

¹Department of Genetics, Program in Cellular Neuroscience, Neurodegeneration and Repair,Yale University School of Medicine

Capítulos

00:05Título
01:02Setting Up the Laser Ablation System
02:17Focusing the Laser: Z-plane
04:12Focusing the Laser: X-Y Place
05:00Immobilizing Worms for Laser Ablation
06:05Performing Laser Axotomies
07:57Scoring Neurons for Regeneration
08:32Conclusion

Summary

Tadução automática

减少神经元的协议<em> C。线虫</em>与MicroPoint脉冲激光。我们描述了建立该系统，固定蠕虫，并切断标记的神经元。优势包括成本相对较低的系统和能力，切断神经元突起或消融细胞<em>在体内</em>。

Tags

In Vivo Laser Axotomy C. Elegans Neurons Regeneration Model Organism Genes Signaling Pathways Laser-mediated Cutting Axotomy Fluorescently-labeled Neuronal Process Femtosecond Laser Pulsed Laser MicroPoint Pulsed Laser Targeted Cell Ablation

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