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In vivo Laser Axotomy in C. elegans
Kapitel
- 00:05Titel
- 01:02Setting Up the Laser Ablation System
- 02:17Focusing the Laser: Z-plane
- 04:12Focusing the Laser: X-Y Place
- 05:00Immobilizing Worms for Laser Ablation
- 06:05Performing Laser Axotomies
- 07:57Scoring Neurons for Regeneration
- 08:32Conclusion
A protocol to cut neurons in C. elegans with a MicroPoint pulsed laser is presented. We describe setting up the system, immobilizing worms, and severing labeled neurons. Advantages include a relatively low-cost system and the ability to sever neuronal processes or ablate cells in vivo.
