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活大鼠皮层神经元线粒体膜电位和活性氧的测定
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
DOI:

09:56 min

May 23, 2011

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Capítulos

  • 00:05Título
  • 01:46Introduction
  • 02:23Preparing the Stock Solutions for the Fluorescent Probes TMRM and H2DCF-DA
  • 03:15Loading Rat Cortical Neurons with TMRM and H2DCF-DA
  • 04:42Live Imaging of Neurons Incubated with TMRM to determine ΔΨm
  • 06:17Live Imaging of Neurons Incubated with H2DCF-DA to Determine ROS
  • 07:18Data Analysis
  • 08:22Representative Results: Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
  • 09:33Conclusion

Summary

Tadução automática

我们证明在皮层神经元的应用荧光指标,TMRM TMRM荧光强度的相对变化,以确定应用程序之前和之后的一个特定的刺激。我们也应用荧光探针H<sub> 2</sub> DCF - DA评估皮层神经元的活性氧物种的相对水平。

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