This content is Free Access.
Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software
27,609 Views
•
08:32 min
•
March 24, 2011
DOI:
Summary
Automatically generated
The C. elegans embryo is a powerful system for studying cell biology and development. We present a protocol for live imaging of C. elegans embryos utilizing DIC optics or fluorescence using readily available epifluorescent microscopes and open-source software.
Explore More Videos
ImagingC. Elegans EmbryosEpifluorescent MicroscopeOpen Source SoftwareTime-lapse ImagingLiving CellsFluorescent-labeled Reporter ProteinsDifferential Interference Contrast MicroscopyDevelopmental RegulationsCell BiologyDevelopmentCaenorhabiditis Elegans EmbryoCell LineageGenetic ManipulationForward GeneticsReverse GeneticsRNAi-mediated InterferenceTargeted MutagenesisTransgenic AnimalsFluorescently Tagged ProteinsCompound Epifluorescent Microscope
Read Article
Cite this Article
Verbrugghe, K. J. C., Chan, R. C. Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software. J. Vis. Exp. (49), e2625, doi:10.3791/2625 (2011).
Copy