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Biologia
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يرتبط فخ كروموسوم لتحديد الحمض النووي تفاعلات طويلة المدى
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Biologia
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JoVE Journal Biologia
Associated Chromosome Trap for Identifying Long-range DNA Interactions
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يرتبط فخ كروموسوم لتحديد الحمض النووي تفاعلات طويلة المدى

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14:49 min

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April 23, 2011

DOI:

10.3791/2621-v

DOI:
10.3791/2621-v

•

14:49 min
April 23, 2011

•

2 Views
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1Medical Service, VA Palo Alto Health Care System ,Stanford University School of Medicine

Summary

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The associated chromosome trap (ACT) assay is a novel unbiased method for identifying long-range DNA interactions. The characterization of long range DNA interactions will allow us to determine the relationship of nuclear architecture to gene expression in both normal physiology and in diseased states.

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Associated Chromosome TrapLong-range DNA InteractionsChromatin StructureChromosome TerritoriesGene ExpressionTranscriptional FactoriesCo-localizationACT AssayChromosome Conformation Capture TechniqueTranscriptional RegulationNuclear ArchitectureGene Expression In Disease States

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Cite this Article

Ling, J., Hoffman, A. R. Associated Chromosome Trap for Identifying Long-range DNA Interactions. J. Vis. Exp. (50), e2621, doi:10.3791/2621 (2011).

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