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An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain
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Neurociência
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JoVE Journal Neurociência
An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

DOI:
10.3791/2486-v

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10:41 min

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December 11, 2010

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, ,
1Department of Molecular Biomedical Sciences, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine,North Carolina State University

Capítulos

  • 00:02Título
  • 00:06Introduction
  • 00:51Preparation of Culture Dishes
  • 01:57Organotypic Slice Preparation
  • 04:19Explant Preparation, Transplantation and Imaging
  • 08:04Representative Results and Analysis
  • 09:16Conclusion

Summary

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This protocol describes an organotypic slice assay optimized for the postnatal brain and high-resolution time-lapse imaging of neuroblast migration in the rostral migratory stream.

Tags

Organotypic Slice AssayHigh-resolution Time-lapse ImagingNeuronal MigrationPostnatal BrainNeurogenesisProgenitor CellsSubependymal Zone (SEZ)Rostral Migratory Stream (RMS)Olfactory Bulbs (OB)Cellular MechanismsMolecular MechanismsPhysiological MechanismsExtracellular ConditionsMigrating NeuroblastsUniform MovementOriented MovementSpeedy MovementCell Autonomous RegulationNon-autonomous RegulationCross-transplantation Approaches

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