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An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain
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Neuroscienze
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JoVE Journal Neuroscienze
An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

11,746 Views

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10:41 min

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December 11, 2010

DOI:

10.3791/2486-v

DOI:
10.3791/2486-v

•

10:41 min
December 11, 2010

•

4 Views
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1Department of Molecular Biomedical Sciences, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine,North Carolina State University

Summary

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This protocol describes an organotypic slice assay optimized for the postnatal brain and high-resolution time-lapse imaging of neuroblast migration in the rostral migratory stream.

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Organotypic Slice AssayHigh-resolution Time-lapse ImagingNeuronal MigrationPostnatal BrainNeurogenesisProgenitor CellsSubependymal Zone (SEZ)Rostral Migratory Stream (RMS)Olfactory Bulbs (OB)Cellular MechanismsMolecular MechanismsPhysiological MechanismsExtracellular ConditionsMigrating NeuroblastsUniform MovementOriented MovementSpeedy MovementCell Autonomous RegulationNon-autonomous RegulationCross-transplantation Approaches

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Jacquet, B. V., Ruckart, P., Ghashghaei, H. T. An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain. J. Vis. Exp. (46), e2486, doi:10.3791/2486 (2010).

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