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Lokalisera Protein i 3D Neural Stem Cell Culture: en hybrid Visualisering Methodology
JoVE Journal
Neurociência
Author Produced
This content is Free Access.
JoVE Journal Neurociência
Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology
DOI:

21:47 min

December 19, 2010

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Capítulos

  • 00:01Título
  • 00:56Introduction
  • 01:37Isolation of Neural Progenitor Cells
  • 05:50Serial Cryosectioning
  • 07:03Immunocytochemistry
  • 08:49Imaging
  • 09:17Alignment
  • 10:23Cell Typology
  • 12:30Cell Mapping
  • 13:48Importing and Assembling Maps in 3-D
  • 15:25Locating Progenitor Cell Typologies in 3-D
  • 19:23Rendering/Compositing
  • 21:05Conclusion

Summary

Tadução automática

Här beskriver vi hur man kan producera, expandera och immunolabel postnatal hippocampus neurala progenitorceller (NPC) i tre-dimensionell (3D) kultur. Nästa, med hjälp av hybrid-visualisering teknik, visar vi hur digitala bilder av immunolabelled kryosnitt kan användas för att rekonstruera och kartlägga de rumsliga position immunopositive celler i hela 3D neurosphere.

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