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Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

DOI:
10.3791/2451-v

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13:05 min

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March 17, 2011

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, , ,
1Department of Biology,University of Iowa, 2Molecular Targeting Technologies,Inc.

Capítulos

  • 00:05Título
  • 01:25Lipophilic Dye Injection
  • 03:32In situ Hybridization
  • 08:00Immunohistochemistry
  • 09:23Histology
  • 11:08Multifactorial Analyses of Neuron Populations in Mutant Mice
  • 12:23Conclusion

Summary

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A combination of different techniques to maximize data collection from mouse tissue is presented.

Tags

Lipophilic DyeIn Situ HybridizationImmunohistochemistryHistologyGene Regulatory NetworksMultiple MutationsWhole Mounted Developing OrgansCellular Expression AlterationsCre Or FlipaseConditional Gene DeletionBreeding SchemesGenetic ModificationsExpression ProfilesHistologically Recognizable StructuresGene And Protein Expression

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