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Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
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Capítulos
- 00:00Título
- 01:15Material Preparation
- 02:41Tissue Preparation
- 04:20Cryopreservation
- 06:05Thaw and Culture
- 08:42Conclusion
Here, we describe a method for efficient cryopreservation and thawing of cortical brain tissue blocks to generate highly enriched neuronal cultures. This simple protocol provides flexibility for later generation of neuronal, astrocyte, and neuronal precursor cell cultures.
Tags
CryopreservationNeuronal CulturesFreezing MediumDimethyl Sulfoxide (DMSO)Hank’s Buffered Salt Solution (HBSS)CryovialsRate-controlled Freezing ContainerPost-thaw ProcessingDissociationNeuronal-enriched CulturesNeuritic GrowthImmunocytochemical StainingAstrocytic MarkerGlial Fibrillary Acidic Protein (GFAP)Neuronal MarkerBeta-tubulin Class IIINeural Precursor Cell CulturesNeurospheresDifferentiating Conditions
