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Immunology and Infection

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JoVE Journal Immunology and Infection
Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites
 
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Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites

Article DOI: 10.3791/2365-v • 13:40 min • January 3rd, 2011
January 3rd, 2011 •
1, 1,2, 1
1National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2School of Life Science, Xiamen University

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Genetic crosses of rodent malaria parasites are performed by feeding two genetically distinct parasites to mosquitoes. Recombinant progeny are cloned from mouse blood after allowing mosquitoes to bite infected mice. This video shows how to produce genetic crosses of Plasmodium yoelii and is applicable to other rodent malaria parasites.

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Genetic Cross Rodent Malaria Parasites Antimalarial Drugs Linkage Mapping Genes Loci Plasmodium Yoelii Laboratory Adaptation Biologic Characteristics Genetic Markers Microsatellite Markers AFLP Markers Plasmodium Falciparum Techniques Drs. David Walliker Richard Carter University Of Edinburgh Mice Mus Musculus Gametocytes Phenotypes Mosquitoes
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