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Organotypic Slice Culture of E18 Rat Brains

JoVE Journal
Biologia

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JoVE Journal Biologia

Organotypic Slice Culture of E18 Rat Brains

Organotypic Slice Culture of E18 Rat Brains

DOI:

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18:48 min

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July 11, 2007

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Laura Elias, Arnold Kriegstein

¹Institute for Regeneration Medicine,University of California, San Francisco – UCSF

Capítulos

00:01Título
00:16Introduction
00:52Tools and Reagents
01:45Preparation
04:28Brain Dissection
06:46Embedding Brains in Agarose
08:57Sectioning Brains
15:01Preparing Brain Slices for Culture
18:08Conclusion
18:31End Credits

Tags

Organotypic Slice Culture E18 Rat Brains Brain Development In-vivo System Manipulations Time-lapse Microscopy Gene Expression Ganglionic Emanances In-utero Electroporation Pharmacological Studies Video Protocol Rat Embryonic Day 18 Embryos Dissecting Embryos Low Melt Agarose Vibratome Plating Slices Filters Culture Dishes

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