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Retrovirale Transduktion von T-Zell-Rezeptoren in Maus-T-Zellen
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Retroviral Transduction of T-cell Receptors in Mouse T-cells

Retrovirale Transduktion von T-Zell-Rezeptoren in Maus-T-Zellen

DOI:

10:14 min

October 22, 2010

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Capítulos

  • 00:00Título
  • 01:37Retroviral Transfection and T-cell Isolation
  • 03:55Prepapration of a Single-cell Suspension from Mouse Spleen
  • 04:51Isolation and Activation of Mouse T-cells
  • 06:27Infection of Activated T-cells
  • 08:21Expansion of Cells and Evaluation of Transduction Efficiency
  • 08:55Retroviral Expression of TCR on Mouse T-cells
  • 09:32Conclusion

Summary

Tadução automática

Wir präsentieren ein Protokoll zur Antigen-spezifischen Maus-T-Zellen produzieren unter Verwendung von retroviralen Transduktion

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