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一个96孔微孔板为基础的方法监测的形成和抗真菌的药敏试验白色念珠菌生物膜
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
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一个96孔微孔板为基础的方法监测的形成和抗真菌的药敏试验白色念珠菌生物膜

DOI:
10.3791/2287-v

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07:19 min

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October 21, 2010

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, , ,
1Department of Biology,University of Texas San Antonio – UTSA, 2South Texas Center for Emerging Infectious Diseases,University of Texas San Antonio – UTSA

Capítulos

  • 00:00Título
  • 01:24Preparation of C. albicans
  • 02:49Setting Up the 96-well Microtiter Plate for the Formation of the Biofilm
  • 03:55Antifungal Susceptibility Testing of Biofilms
  • 06:02Evaluation of Antifungal Reagents
  • 06:43Conclusion

Summary

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我们描述了一个简单，快速和可靠的方法形成<em>白色念珠菌</em>生物膜在细胞内生物膜的抗真菌药敏试验使用96孔板和它的效用。

Tags

96 Well Microtiter PlateMonitoringFormationAntifungal Susceptibility TestingCandida AlbicansBiofilmsCandidiasisFungal InfectionsMedical DevicesCathetersIn Vitro ModelColorimetric AssayXTTTetrazolium SaltMetabolically Active Fungal Biofilm CellsExperiment DurationAntifungal Susceptibility Testing Standardization

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