Recording of Local Field Potential in Mouse Hippocampal-Entorhinal Cortex Slices
Transcrição
To record local field potentials, fill a 400-milliliter beaker with carbogen-bubbled ACSF, and place one end of the pump tubing into the beaker. Turn on a peristaltic pump at eight to 10 milliliters per minute, to direct ACSF from the 400-milliliter beaker to a heated reservoir and from the reservoir to the recording chamber at 32 degrees Celsius.
Next, briefly clamp the tubing, and turn off the pump to pause the flow. Using fine forceps, transfer a brain tissue slice to the recording chamber by the corner of the lens paper the tissue is resting on, slice down. Peel away the lens paper, leaving the slice submerged in the recording chamber, and use a harp to secure the slice.
Using a manual micromanipulator, slowly advance the tip of a sodium chloride-filled stimulation pipette into the surface of the slice at a 30- to 45-degree angle. Then, use a second micromanipulator to slowly advance the tip of an ACSF-filled local field potential pipette into the region of interest at a 30- to 45-degree angle, and record the local field potential of the sample according to standard protocols.
