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Employing Reporter Cells in a Co-Culture to Study Retinoic Acid Production by Mouse Embryonic Cells
Transcrição
For embryonic cell co-culturing, begin dissection of E8.5 embryos. Next, load a new 1.5-milliliter tube with 10 microliters of trypsin, and using a widened-bore P20 pipette tip, transfer the whole embryo into the tube, and place the tube on ice.
After all the embryos have been isolated, incubate all the tubes at 37 degrees Celsius for five minutes to dissociate the tissues. After 5 minutes, gently triturate each tube's contents using P20 pipette tip and transfer 10 microliters to a well of the prepared 96-well plate with F9 RARE LacZ cells. Culture the plate overnight.
