This content is Free Access.
Capítulos
- 00:04Título
- 00:45Introduction
- 01:33Preparing Geltrex-coated Culture Dishes
- 02:59Adapting Human iPSCs
- 05:20Passaging Human iPSCs
- 07:36Representative Results
- 08:07Conclusion
Följande protokoll innehåller instruktioner för att anpassa människa inducerade pluripotenta stamceller (IPS) celler till feeder-fri kultur med hjälp av kompletta Knockout Serum Byte Feeder-Free medium (KSR-FF). När anpassad, instruktioner för kontinuerlig underhåll finns också.
Tags
Feeder-Free AdaptationCulturePassagingHuman IPS CellsComplete KnockOut Serum ReplacementFeeder-Free MediumReprogrammedIPS CellsEmbryonic Stem CellsPluripotent CellsDrug DiscoveryCell TherapyBasic ResearchGIBCO Media And ReagentsKnockout DMEMKnockout Serum ReplacementIPS Cell CultureFeeder-free CultureKnockout SR Growth Factor CocktailTraditional Culture MethodsMouse Fibroblast Feeder LayersHuman Fibroblast Feeder LayersFeeder-conditioned MediumLabor-intensiveHard To ScaleUndifferentiated HiPS CellsUndefined Conditions
