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免费接驳适应，文化和传代使用完整的基因敲除血清替代馈线中的人类iPS细胞
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
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免费接驳适应，文化和传代使用完整的基因敲除血清替代馈线中的人类iPS细胞

DOI:
10.3791/2236-v

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08:29 min

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July 15, 2010

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,
1GIBCO,Life Technologies

Capítulos

  • 00:04Título
  • 00:45Introduction
  • 01:33Preparing Geltrex-coated Culture Dishes
  • 02:59Adapting Human iPSCs
  • 05:20Passaging Human iPSCs
  • 07:36Representative Results
  • 08:07Conclusion

Summary

Tadução automática

Tadução automática

以下协议提供馈线自由的文化，使用完整的基因敲除血清替代馈线培养基（KSR - FF）为适应人类诱导多能干细胞（iPS）细胞的指令。一旦适应，也提供了不断维护的说明。

Tags

Feeder-Free AdaptationCulturePassagingHuman IPS CellsComplete KnockOut Serum ReplacementFeeder-Free MediumReprogrammedIPS CellsEmbryonic Stem CellsPluripotent CellsDrug DiscoveryCell TherapyBasic ResearchGIBCO Media And ReagentsKnockout DMEMKnockout Serum ReplacementIPS Cell CultureFeeder-free CultureKnockout SR Growth Factor CocktailTraditional Culture MethodsMouse Fibroblast Feeder LayersHuman Fibroblast Feeder LayersFeeder-conditioned MediumLabor-intensiveHard To ScaleUndifferentiated HiPS CellsUndefined Conditions

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