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An In Vitro Assay for Screening Interferons as Potent Inhibitors of Zika Virus Growth

An In Vitro Assay for Screening Interferons as Potent Inhibitors of Zika Virus Growth

Transcrição

Six hours after seeding a 12-well plate with naïve Vero cells, add cytokines of interest at indicated concentrations in biological duplicates. 12 hours post-treatment, perform Zika virus infection, and include negative control wells without infection. Four hours after infection, replace the viral inoculum with cytokine-treated medium, and then incubate the cells at 37 degree Celsius for an additional 44 hours. At 48 hours post-infection, count viral plaques using a phase-contrast microscope, and acquire representative images of infected cells at 40x magnification.

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