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Determining Cell Number During Cell Culture using the Scepter Cell Counter
JoVE Journal
Biologia
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JoVE Journal Biologia
Determining Cell Number During Cell Culture using the Scepter Cell Counter

Determining Cell Number During Cell Culture using the Scepter Cell Counter

DOI:
10.3791/2204-v

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09:33 min

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November 26, 2010

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, , , ,
1Bioscience Division,Millipore Inc

Capítulos

  • 00:00Título
  • 00:50The Importance of Cell Counting
  • 02:57Preparation of Single-cell Suspension for Counting
  • 03:23Performing Cell Counts Using the Scepter
  • 05:16Data Analysis
  • 06:41Linearity and Precision of Scepter Cell Counting Across Multiple Cell Lines
  • 09:16Conclusion

Summary

Tadução automática

Tadução automática

The Scepter Cell Counter is a handheld automated device that can be used to count cells, monitor cell diameter and volume, and be used to check the health and quality of cellular populations from one culture to the next.

Tags

Cell CultureCell CounterCell CountingIn VitroExperimental ReproducibilityAccuracyCell HealthProliferation RateImmortalizationTransformationSeeding CellsTransfectionInfectionCell-based AssaysHemocytometerUser BiasMisuseInaccurate CountsOptical GlassMicroscopeErrors In HemocytometryAutomated Cell Counting Devices

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