Journal
/
Biología
/
Determining Cell Number During Cell Culture using the Scepter Cell Counter
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Determining Cell Number During Cell Culture using the Scepter Cell Counter

Determining Cell Number During Cell Culture using the Scepter Cell Counter

DOI:
10.3791/2204-v

•

09:33 min

•

November 26, 2010

•

, , , ,
1Bioscience Division,Millipore Inc

Capítulos

  • 00:00Título
  • 00:50The Importance of Cell Counting
  • 02:57Preparation of Single-cell Suspension for Counting
  • 03:23Performing Cell Counts Using the Scepter
  • 05:16Data Analysis
  • 06:41Linearity and Precision of Scepter Cell Counting Across Multiple Cell Lines
  • 09:16Conclusion

Summary

Traducción Automática

Traducción Automática

The Scepter Cell Counter is a handheld automated device that can be used to count cells, monitor cell diameter and volume, and be used to check the health and quality of cellular populations from one culture to the next.

Tags

Cell CultureCell CounterCell CountingIn VitroExperimental ReproducibilityAccuracyCell HealthProliferation RateImmortalizationTransformationSeeding CellsTransfectionInfectionCell-based AssaysHemocytometerUser BiasMisuseInaccurate CountsOptical GlassMicroscopeErrors In HemocytometryAutomated Cell Counting Devices

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

Read Article