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Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
JoVE Journal
Biologia
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JoVE Journal Biologia
Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

DOI:
10.3791/2160-v

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11:00 min

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September 09, 2010

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, ,
1Department of Molecular and Cellular Biology,University of Guelph

Capítulos

  • 00:00Título
  • 00:27Introduction
  • 02:59Materials & Methods
  • 09:36Conclusion

Summary

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In this video we use an adenovirus carrying the Cre recombinase gene to infect primary mouse embryonic fibroblasts carrying a floxed Rac1 allele.

Tags

Adenovirus-mediatedGenetic RemovalSignaling MoleculesCultured Primary Mouse Embryonic FibroblastsCre-loxP SystemConditional DeletionCre RecombinaseFloxed DNARecombination EventExcisionAdenovirusCre Recombinase GeneInfectPrimary Mouse Embryonic Fibroblasts (MEFs)Floxed Rac1 AlleleMorphological ChangesActin CytoskeletonViral TransductionCre ExpressionStained Using Phalloidin DyeConfocal Laser Scanning Microscopy

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