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Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations
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Neurociência
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JoVE Journal Neurociência
Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations

Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations

DOI:
10.3791/2128-v

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07:18 min

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September 29, 2010

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, , ,
1Department of Biological Sciences,SUNY-University at Buffalo

Capítulos

  • 00:00Título
  • 00:49Introduction
  • 01:10Dissection of 3rd Instar Larvae
  • 03:20Fixation and Antibody Staining of the Dissected Larva
  • 05:06Mounting and Visualization of the Dissected Larva
  • 06:17Representative Images of Drosophila Larvae Segmental Nerves
  • 06:49Conclusion

Summary

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Drosophila melanogaster larvae provide an ideal model system to investigate the mechanisms of axonal transport within larval segmental nerves. Using this procedure, 3rd instar larvae carrying various mutations can be compared to wild type larvae.

Tags

Larval Segmental Nerves3rd Instar Drosophila LarvaeNervous System DevelopmentAxonal TransportSynaptic Vesicle ProteinsVisualizedLarval PreparationsDrosophila MelanogasterGenéticaFully Sequenced GenomeAxonsCell BodiesNerve TerminalsSynaptic Vesicle AntibodiesWild Type Larvae

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