A Thin-skull Window Technique for Chronic Two-photon <em>In vivo</em> Imaging of Murine Microglia in Models of Neuroinflammation

Daniel F. Marker; Marie-Eve Tremblay; Shao-Ming Lu; Ania K. Majewska; Harris A. Gelbard

doi:10.3791/2059

Journal

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Neurociência

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Kronik İki foton için İnce kafatası Pencere Tekniği In vivo Görüntüleme

JoVE Journal
Neurociência

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JoVE Journal Neurociência

A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation

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Kronik İki foton için İnce kafatası Pencere Tekniği In vivo Görüntüleme

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12:07 min

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September 19, 2010

DOI:

10.3791/2059-v

DOI:

10.3791/2059-v

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12:07 min

September 19, 2010

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Daniel F. Marker*¹, Marie-Eve Tremblay*², Shao-Ming Lu, Ania K. Majewska, Harris A. Gelbard

¹Center for Neural Development and Disease, Department of Neurology, Child Neurology Division,University of Rochester, ²Department of Neurobiology and Anatomy,University of Rochester

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Thin-skull Window Technique Chronic Two-photon In Vivo Imaging Murine Microglia Neuroinflammation Open-skull Technique Thinned-skull Approach Microglial Activation Chronic Imaging Imaging Sessions Two-photon Microscopy Stable Cortical Window Glass Coverslip Translucent Immunologically Inert CX3CR1 GFP/+ Mice Pial Surface Stereotactic Brain Injury

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Marker, D. F., Tremblay, M., Lu, S., Majewska, A. K., Gelbard, H. A. A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation. J. Vis. Exp. (43), e2059, doi:10.3791/2059 (2010).

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