A Thin-skull Window Technique for Chronic Two-photon <em>In vivo</em> Imaging of Murine Microglia in Models of Neuroinflammation

Daniel F. Marker; Marie-Eve Tremblay; Shao-Ming Lu; Ania K. Majewska; Harris A. Gelbard

doi:10.3791/2059

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A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation

JoVE Journal
神経科学

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JoVE Journal 神経科学

A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation

A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation

23,108 Views

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12:07 min

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September 19, 2010

DOI:

10.3791/2059-v

DOI:

10.3791/2059-v

•

12:07 min

September 19, 2010

•

11 Views

Daniel F. Marker*¹, Marie-Eve Tremblay*², Shao-Ming Lu¹, Ania K. Majewska², Harris A. Gelbard¹

¹Center for Neural Development and Disease, Department of Neurology, Child Neurology Division,University of Rochester, ²Department of Neurobiology and Anatomy,University of Rochester

概要

We describe a method for repeatedly visualizing murine microglia and circulating monocytes in vivo over hours, days or weeks using transcranial two-photon microscopy. We demonstrate how to prepare a thinned-skull window that allows intermittent observation of quiescent microglia that can be activated by adjacent stereotactic injection of the HIV-1 regulatory protein Tat.

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A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation

概要

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