Journal
/
Biologia
/
Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

11,855 Views

•

05:45 min

•

April 26, 2010

DOI:

10.3791/2012-v

DOI:
10.3791/2012-v

•

05:45 min
April 26, 2010

•

2 Views
, , ,
1Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Department of Physics, Lewis-Sigler Institute for Integrative Genomics,Princeton University

Summary

Automatically generated

We present a protocol for bending filamentous bacterial cells attached to a cover-slip surface with an optical trap to measure the cellular bending stiffness.

Explore More Videos

MeasuringBending StiffnessBacterial CellsOptical TrapProtocolFilamentous Rod-shaped BacteriaForcesMicroscopic Three-dimensional SpringHigh-intensity Laser BeamChemically-treated CoverslipLive BacteriaGrowing EndsFilamentous GrowthDrug CephalexinCell AttachmentBending ForcesPolylysine-coated BeadForce MeasurementDisplacement Measurement

Read Article

Cite this Article

Wang, S., Arellano-Santoyo, H., Combs, P. A., Shaevitz, J. W. Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap. J. Vis. Exp. (38), e2012, doi:10.3791/2012 (2010).

Download .ris file

Copy

Share Video

.

Copy