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Suspension-Trapping Sample Preparation of Human Tear Protein (Video) | JoVE
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Suspension-Trapping Sample Preparation of Human Tear Protein

Suspension-Trapping Sample Preparation of Human Tear Protein (Video) | JoVE

Non-Invasive Biomarker Discovery for Tear Proteomics Using LC-MS

DOI:
10.3791/200490-v

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03:45 min

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December 01, 2023

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, , , ,
1Centre for Myopia Research, School of Optometry,The Hong Kong Polytechnic University, 2Centre for Eye and Vision Research (CEVR), 3Research Centre for SHARP Vision (RCSV),The Hong Kong Polytechnic University, 4Research Centre for Chinese Medicine Innovation (RCMI),The Hong Kong Polytechnic University

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Keywords: Suspension-trappingTear ProteinSample PreparationDithiothreitolIodoacetamidePhosphoric AcidProtein Binding BufferTEAB Digestion BufferFormic AcidAcetonitrileGene Ontology AnalysisProteomeData Dependent Acquisition

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