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Unveiling Mitochondrial Ultrastructure in Neurons Using STED Microscopy

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Imaging SH-SY5Y Cells by STED Microscopy and Subsequent Analysis of Mitochondrial Ultrastructure

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

Imaging SH-SY5Y Cells by STED Microscopy and Subsequent Analysis of Mitochondrial Ultrastructure

Imaging SH-SY5Y Cells by STED Microscopy and Subsequent Analysis of Mitochondrial Ultrastructure

DOI:

10.3791/200327-v

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04:05 min

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June 30, 2023

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Emery L. Ng, Ashley L. Reed, Christopher B. O’Connell, Nathan N. Alder

¹Center for Open Research Resources and Equipment,University of Connecticut, ²Department of Molecular and Cell Biology,University of Connecticut

Tags

STED Microscopy Mitochondrial Ultrastructure SH-SY5Y Cells LightBox Software Confocal Acquisition Settings STED Acquisition Deconvolution Trainable Weka Segmentation Cristae Image Analysis

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