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Preparation of Drosophila S2 cells for Light Microscopy
JoVE Journal
Biologia
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JoVE Journal Biologia
Preparation of Drosophila S2 cells for Light Microscopy
DOI:

09:39 min

June 03, 2010

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Capítulos

  • 00:00Título
  • 00:51Introduction
  • 01:28Preparation of Glass-Bottom Dishes
  • 04:16Preparation of S2 Cells for Live Cell Microscopy
  • 05:12Fixation and Immunostaining of S2 Cells in the Glass-bottom Dish
  • 07:50Results of Microscopy of S2 Cells
  • 09:21Conclusion

Summary

Tadução automática

Drosophila Schneider (S2) cells are an increasingly popular system for the discovery and functional analysis of genes. Our goal is to describe some of the microscopic techniques that make S2 cells such an increasingly important experimental system.

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