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Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun
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Biologia
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JoVE Journal Biologia
Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

DOI:
10.3791/1963-v

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10:39 min

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June 12, 2010

•

,
1Dept. Of Cell Biology and Molecular Genetics,University of Maryland

Capítulos

  • 00:00Título
  • 00:57Introduction
  • 01:24Preparing for Bombarding the Cells
  • 03:32Bombardment
  • 07:16Observation
  • 08:50Representative Images of the BiFC Assay in Onion Cells Transfected with the Helios Gene Gun
  • 10:06Conclusion

Summary

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Tadução automática

This article illustrates how to properly use the BioRad Helios Gene Gun to introduce plasmid DNA into onion epidermal cells and how to test for protein-protein interactions in onion cells based on the principle of Bimolecular Fluorescence Complementation (BiFC)

Tags

Bimolecular Fluorescence ComplementationBiFC AssayProtein-protein InteractionOnion CellsHelios Gene GunGene FunctionFluorescent ProteinGFPPlant ResearchersOnion Epidermal CellsPlasmid DNAArabidopsis ThalianaSEUSSLEUNIG HOMOLOG

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