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siRNAトランスフェクション後の有糸分裂のタイムラプスイメージング

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Time-lapse Imaging of Mitosis After siRNA Transfection

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siRNAトランスフェクション後の有糸分裂のタイムラプスイメージング

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08:21 min

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June 06, 2010

DOI:

DOI:

•

08:21 min

June 06, 2010

•

3 Views

Douglas R. Mackay¹, Katharine S. Ullman¹, Christopher K. Rodesch²

¹Department of Oncological Sciences, Huntsman Cancer Institute,University of Utah, ²Fluorescence Microscopy Core Facility,University of Utah

Summary

ここでは、画像への基本的なプロトコルを記述し、siRNAのトランスフェクション後のライブ哺乳類の組織培養細胞の有糸分裂のタイミングを定量化する。

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