Time-lapse Imaging of Mitosis After siRNA Transfection

Douglas R. Mackay; Katharine S. Ullman; Christopher K. Rodesch

doi:10.3791/1878

Journal

/

Biologia

/

Zeitraffer-Imaging der Mitose Nach siRNA-Transfektion

JoVE Journal
Biologia

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JoVE Journal Biologia

Time-lapse Imaging of Mitosis After siRNA Transfection

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Zeitraffer-Imaging der Mitose Nach siRNA-Transfektion

16,628 Views

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08:21 min

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June 06, 2010

DOI:

10.3791/1878-v

DOI:

10.3791/1878-v

•

08:21 min

June 06, 2010

•

3 Views

Douglas R. Mackay¹, Katharine S. Ullman¹, Christopher K. Rodesch²

¹Department of Oncological Sciences, Huntsman Cancer Institute,University of Utah, ²Fluorescence Microscopy Core Facility,University of Utah

Summary

Hier beschreiben wir eine grundlegende Protokoll, um Bild und Quantifizierung der mitotischen Zeitpunkt der Live-Säugetier-Zellkultur-Zellen nach siRNA-Transfektion.

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Time-lapse Imaging Mitosis SiRNA Transfection Cellular Organization Chromosome Dynamics Fluorescence Microscopy GFP-tagged Proteins RNAi-based Depletion Mitotic Regulatory Protein Automated Fluorescence Microscope Image Sequences Still-image Montages Movies Quantification Of Mitotic Stages MCherry-tagged Histone H2B Time-lapse Experiment Design

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Zeitraffer-Imaging der Mitose Nach siRNA-Transfektion

Summary

Automatically generated

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