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Utilisation du système d'électroporation Gene Pulser MXcell à transfecter des cellules primaires à haut rendement

JoVE Journal
Biologia

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JoVE Journal Biologia

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

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Utilisation du système d'électroporation Gene Pulser MXcell à transfecter des cellules primaires à haut rendement

23,629 Views

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12:55 min

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January 07, 2010

DOI:

DOI:

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12:55 min

January 07, 2010

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3 Views

Adam M. McCoy, Michelle L. Collins, Luis A. Ugozzoli

¹Gene Expression Division,Bio-Rad Laboratories, Inc.

Tags

Gene Pulser MXcell Electroporation System Transfect Primary Cells High Efficiency Plasmid DNA SiRNA Electroporation Buffer Bio-Rad Nucleic Acids Mammalian Cells Difficult-to-transfect Cells Stem Cells Electroporation Conditions Optimization 96-well Electroporation Plates Electroporation Cuvettes Electroporation Efficiency Success Or Failure Of Electroporation Experiments

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