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Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
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12:55 min
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January 07, 2010
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概要
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This procedure shows how to use the Gene Pulser MXcell electroporation system to rapidly and easily identify the best electroporation conditions for mouse embryonic fibroblasts (MEFs) or other primary cells. Considerations for troubleshooting are also discussed in the associated video.
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McCoy, A. M., Collins, M. L., Ugozzoli, L. A. Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency. J. Vis. Exp. (35), e1662, doi:10.3791/1662 (2010).
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