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Culture and Maintenance of Human Embryonic Stem Cells
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Biologia
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JoVE Journal Biologia
Culture and Maintenance of Human Embryonic Stem Cells

Culture and Maintenance of Human Embryonic Stem Cells

47,119 Views

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09:36 min

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December 22, 2009

DOI:

10.3791/1427-v

DOI:
10.3791/1427-v

•

09:36 min
December 22, 2009

•

15 Views
1Research and Development,Stemgent

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This protocol demonstrates how to maintain healthy, undifferentiated human embryonic stem (ES) cells.

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Human Embryonic Stem CellsHES CellsCulture MaintenanceUndifferentiated CulturesComplete Medium ChangeNutrient ReplenishmentUnwanted Differentiation FactorsDifferentiation CleanupPicking Differentiated AreasExcess Differentiation RemovalHealthy Cell Population Maintenance

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Cite this Article

Kent, L. Culture and Maintenance of Human Embryonic Stem Cells . J. Vis. Exp. (34), e1427, doi:10.3791/1427 (2009).

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