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Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
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Biologia
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JoVE Journal Biologia
Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

DOI:
10.3791/1325-v

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19:16 min

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August 05, 2009

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,
1Department of Cell Biology,Scripps Institute

Capítulos

  • 00:05Título
  • 00:58Introduction
  • 01:24Preparing Fluorescently Labeled Actin and Cells for Microinjection
  • 05:46Microinjection of Labeled Actin
  • 10:14Assembling the Imaging Chamber
  • 15:20Imaging Cells
  • 18:17Representative Results: Microinjected Cells
  • 18:44Conclusion

Summary

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Tadução automática

Selection, microinjection, and imaging of fluorescently-labeled F-actin via fluorescent speckle microscopy (FSM).

Tags

Fluorescent Speckle MicroscopyF-actin DynamicsLive Cell ImagingFSMActin DynamicsPtK1 CellsTurnover KineticsAssembly/disassemblyComputer Vision SoftwareQFSMFluorescent Actin ProbesMacromolecular AssembliesMicrotubulesIntermediate FilamentsAdhesion Complexes

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