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Capítulos
- 00:00Título
- 00:15Introduction
- 01:52Step 1: Collect Embryos
- 02:24Step 2: Prepare a Dechorionation Slide
- 02:51Step 3: Prepare Imaging Chamber
- 03:32Step 4: Place Halocarbon Oil on a Coverslip
- 04:05Step 5: Picking Embryos From Plates
- 04:38Step 6: Place Embryos onto Tape
- 05:01Step 7: Roll Embryos to Remove the Chorion
- 06:15Step 8: Transfer Embryos to Halocarbon Oil
- 06:47Step 9: Push Embryos to Bottom of Oil
- 07:14Step 10: Invert Coverslip on Live Imaging Chamber
- 07:38Step 11: Secure Coverslip with Tape
A simple, inexpensive, and effective method of preparing Drosophila embryos for live-imaging analysis is presented. Our protocol provides humidity and gas exchange and does not compress the Drosophila embryo. This method is suitable for GFP-based live imaging of Drosophila embryos using a stereomicroscope or upright compound microscope.
