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Monitoring Plant Hormones During Stress Responses
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JoVE Journal Biologia
Monitoring Plant Hormones During Stress Responses

Monitoring Plant Hormones During Stress Responses

DOI:
10.3791/1127-v

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11:01 min

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June 15, 2009

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,
1Department of Biology,University of Texas

Capítulos

  • 00:05Título
  • 00:39Introduction
  • 01:10Extraction of Plant Material
  • 04:16Methylation and Preparation of Filters
  • 06:43Collecting Plant Extraction
  • 08:10Analysis of Samples
  • 09:58Representative Results/ Outcome
  • 10:30Conclusion

Summary

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A simple method is provided that allows for the rapid extraction and analysis of multiple plant hormones from small tissue samples. The procedure uses vapor phase extraction as the solemn purification step. Samples are analyzed by GC/MS with chemical ionization that produces mainly (M+1)+ ions.

Tags

Plant HormonesStress ResponsesEnvironmental StimuliInsect HerbivoryPathogen InfectionDrought StressJasmonic Acid (JA)Salicylic Acid (SA)Abscisic Acid (ABA)Temporal MonitoringSpatial MonitoringVapor Phase ExtractionGas Chromatography/mass Spectrometry (GC/MS) AnalysisQuantificationInternal Standards

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