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Microinjection of Zebrafish Embryos to Analyze Gene Function
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Biologia
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JoVE Journal Biologia
Microinjection of Zebrafish Embryos to Analyze Gene Function

Microinjection of Zebrafish Embryos to Analyze Gene Function

76,450 Views

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07:18 min

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March 09, 2009

DOI:

10.3791/1115-v

DOI:
10.3791/1115-v

•

07:18 min
March 09, 2009

•

49 Views
, ,
1Department of Genetics,Harvard Medical School, 2Department of Cardiology,Children’s Hospital Boston

Summary

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This video shows how morpholino or mRNA can be injected into zebrafish embryos at the one-cell stage to decrease or increase the level of specific gene products during subsequent development.

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MicroinjectionZebrafish EmbryosGene Function AnalysisProtein ManipulationMorpholinosAntisense OligonucleotidesGene ExpressionMRNA InjectionMicroinjection ProcessEmbryo ManipulationInjection Volume

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Cite this Article

Rosen, J. N., Sweeney, M. F., Mably, J. D. Microinjection of Zebrafish Embryos to Analyze Gene Function. J. Vis. Exp. (25), e1115, doi:10.3791/1115 (2009).

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