Application of Monolayer Graphene to Cryo-Electron Microscopy Grids for High-resolution Structure Determination

Andrew V. Grassetti; Mira B. May; Joseph  H. Davis

doi:10.3791/66023

JoVE Journal > Biochemistry

Bioquímica

Application of Monolayer Graphene to Cryo-Electron Microscopy Grids for High-resolution Structure Determination

Published: November 10, 2023

doi:

10.3791/66023

Andrew V. Grassetti*¹, Mira B. May*¹, Joseph H. Davis^1,2

¹Department of Biology,Massachusetts Institute of Technology, ²Program in Computational and Systems Biology,Massachusetts Institute of Technology

Summary

The application of support layers to cryogenic electron microscopy (cryoEM) grids can increase particle density, limit interactions with the air-water interface, reduce beam-induced motion, and improve the distribution of particle orientations. This paper describes a robust protocol for coating cryoEM grids with a monolayer of graphene for improved cryo-sample preparation.

Abstract

In cryogenic electron microscopy (cryoEM), purified macromolecules are applied to a grid bearing a holey carbon foil; the molecules are then blotted to remove excess liquid and rapidly frozen in a roughly 20-100 nm thick layer of vitreous ice, suspended across roughly 1 µm wide foil holes. The resulting sample is imaged using cryogenic transmission electron microscopy, and after image processing using suitable software, near-atomic resolution structures can be determined. Despite cryoEM's widespread adoption, sample preparation remains a severe bottleneck in cryoEM workflows, with users often encountering challenges related to samples behaving poorly in the suspended vitreous ice. Recently, methods have been developed to modify cryoEM grids with a single continuous layer of graphene, which acts as a support surface that often increases particle density in the imaged area and can reduce interactions between particles and the air-water interface. Here, we provide detailed protocols for the application of graphene to cryoEM grids and for rapidly assessing the relative hydrophilicity of the resulting grids. Additionally, we describe an EM-based method to confirm the presence of graphene by visualizing its characteristic diffraction pattern. Finally, we demonstrate the utility of these graphene supports by rapidly reconstructing a 2.7 Å resolution density map of a Cas9 complex using a pure sample at a relatively low concentration.

Introduction

Single particle cryogenic electron microscopy (cryoEM) has evolved into a widely used method for visualizing biological macromolecules¹. Fueled by advances in direct electron detection²^,³^,⁴, data acquisition⁵, and image processing algorithms⁶^,⁷^,⁸^,⁹^,¹⁰, cryoEM is now capable of producing near-atomic resolution 3D structures of a fast-growing number of macromolecules¹¹. Moreover, by leveraging the single-molecule nature of the approach, users can determine multiple structures from a single sample¹²^,¹³^,¹⁴^,¹⁵, highlighting the promise of using the data generated to understand heterogeneous structural ensembles¹⁶^,¹⁷. Despite this progress, bottlenecks in cryo-specimen grid preparation persist.

For structural characterization by cryoEM, biological samples should be well-dispersed in aqueous solution and then must be flash-frozen through a process called vitrification¹⁸^,¹⁹. The goal is to capture particles in a uniformly thin layer of vitrified ice suspended across regularly spaced holes that are typically cut into a layer of amorphous carbon. This patterned amorphous carbon foil is supported by a TEM grid bearing a mesh of copper or gold support bars. In standard workflows, grids are rendered hydrophilic using a glow-discharge plasma treatment prior to the application of sample. Excess liquid is blotted with filter paper, allowing the protein solution to form a thin liquid film across the holes that can be readily vitrified during plunge-freezing. Common challenges include particle localization to the air-water interface (AWI) and subsequent denaturation²⁰^,²¹^,²² or adoption of preferred orientations²³^,²⁴^,²⁵, particle adherence to the carbon foil rather than migrating into the holes, and clustering and aggregation of the particles within the holes²⁶. Nonuniform ice thickness is another concern; thick ice can result in higher levels of background noise in the micrographs due to increased electron scattering, whereas extremely thin ice can exclude larger particles²⁷.

To address these challenges, a variety of thin support films have been used to coat grid surfaces, allowing particles to rest on these supports and, ideally, avoid interactions with the air-water interface. Graphene supports have shown great promise, in part due to their high mechanical strength coupled with their minimal scattering cross-section, which reduces the background signal added by the support layer²⁸. In addition to its minimal contribution to background noise, graphene also exhibits remarkable electrical and thermal conductivity²⁹. Graphene and graphene oxide coated grids have been shown to yield higher particle density, more uniform particle distribution³⁰, and reduced localization to the AWI²². In addition, graphene provides a support surface that can be further modified to: 1) tune the physiochemical properties of the grid surface through functionalization³¹^,³²^,³³; or 2) couple linking agents that facilitate affinity purification of proteins of interest³⁴^,³⁵^,³⁶.

In this article, we have modified an existing procedure for coating cryoEM grids with a single uniform layer of graphene³⁰. The modifications aim to minimize grid handling throughout the protocol, with the goal of increasing yield and reproducibility. Additionally, we discuss our approach to evaluate the efficacy of various UV/ozone treatments in rendering grids hydrophilic prior to plunging. This step in cryoEM sample preparation using graphene-coated grids is critical, and we have found our straightforward method to quantify the relative hydrophilicity of the resulting grids to be useful. Using this protocol, we demonstrate the utility of employing graphene-coated grids for structure determination by generating a high-resolution 3D reconstruction of catalytically inactive S. pyogenes Cas9 in complex with guide RNA and target DNA.

Protocol

1. Preparation of CVD graphene

Prepare graphene etching solution as described below.
1. Dissolve 4.6 g ammonium persulfate (APS) in 20 mL of molecular grade water in a 50 mL beaker for a 1 M solution and cover with aluminum foil. Allow APS to completely dissolve while proceeding to step 1.2.
Prepare a section of CVD graphene for methyl methacrylate (MMA) coating. Carefully cut a square section of CVD graphene. Transfer the square to a coverslip (50 mm x 24 mm) within a clean Petri dish and cover during transport to the spin coater.
NOTE: An 18 mm x 18 mm square should yield 25-36 graphene-coated grids.

2. Coating CVD graphene with MMA

Set the spin coater settings to a 60 s high speed spin at 2,500 rpm. Carefully place the CVD graphene on the appropriately sized chuck.
NOTE: Ideally, the CVD graphene will extend 1-2 mm over the edge of the selected chuck to prevent aspiration of MMA into the vacuum system.
Press the Take/Absorb button to engage the vacuum pump and affix the CVD graphene to the chuck. Apply MMA to the center of CVD graphene square, close lid, and press Start/Stop button immediately. For an 18 mm x 18 mm square of CVD graphene, 40 µL of MMA is sufficient.
Once spinning has stopped, disengage the vacuum pump and carefully retrieve the MMA coated CVD graphene with tweezers. Invert the CVD graphene such that the MMA coated side is facing down and place it back on the glass coverslip.

3. Plasma etching of the graphene back-side

Transfer CVD graphene on the coverslip into the glow discharger and glow discharge for 30 s at 25 mA using flat-tip tweezers.
Return the CVD graphene on the coverslip to the Petri dish and cover during transport to the copper etching area. MMA coating will protect the topside graphene layer from plasma etching.

4. Cutting grid-sized MMA coated CVD graphene squares

Use two pairs of tweezers to support the CVD graphene square. Take note of the orientation of the CVD graphene square, keep the MMA side up while attached to the tweezers (CRITICAL).
Cut CVD graphene into approximately 3 mm x 3 mm squares. Use two sets of reverse-action forceps for this step to hold the CVD graphene square right-side-up and anchor it in position, and also to hold the edge of a 3 mm x 3 mm square after cutting it away from the rest of the square.

5. Dissolving copper substrate from MMA coated CVD graphene

Carefully float each 3 mm x 3 mm square in the APS solution. Make contact with the surface of the APS solution prior to releasing each square. Tilt the beaker such that each square can be placed in the solution at a shallow angle; this ensures that the square does not sink.
Cover beaker with aluminum foil and incubate overnight at 25 °C.

6. Removing MMA/graphene films from APS

Use a glass coverslip with dimensions 12 mm x 50 mm and extract the MMA/graphene squares from APS by gently plunging the coverslip vertically into the APS and then moving the coverslip laterally such that it abuts a floating MMA/graphene square.
Carefully remove the coverslip and ensure that the square adheres completely to the side of the coverslip upon removal.
Transfer MMA/graphene squares to a clean 50 mL beaker filled with molecular grade water for 20 min. Gently plunge the coverslip with an MMA/graphene square attached into the water vertically and ensure that the square dislodges from the coverslip upon interaction with the surface of the water. Repeat for all MMA/graphene squares.

7. Adhering graphene to grids

Using negative action tweezers, gently dip a grid vertically into the water with the carbon side facing a floating MMA/graphene square. Once in contact with the square, carefully remove the grid and ensure that the square adheres completely to the carbon side of the grid upon removal. Minimize lateral movement of the grid when submerged in water to prevent damaging grid squares (CRITICAL).
Place grids on a clean coverslip MMA/graphene side up and air dry for 1 to 2 min. Transfer coverslip graphene/MMA coated grids to a hot plate set to 130 °C using flat-tip tweezers.
Cover with the top of a glass Petri dish and incubate for 20 min. Remove from heat and cool to room temperature for 1 to 2 min.
CAUTION: Exercise caution as the Petri dish top will be extremely hot.

8. Dissolving MMA with acetone

Transfer entire coverslip into a Petri dish filled with 15 mL of acetone. Incubate for 30 min.
Using a clean glass serological pipette, transfer acetone, in which the grids were incubated, to a waste container. Carefully add 15 mL of fresh acetone to the Petri dish using a clean glass serological pipette. Incubate for 30 min.
Repeat these wash steps for a total of 3 acetone washes.

9. Removing residual acetone with isopropanol

Following 3 acetone washes, remove acetone and replace with 15 mL of isopropanol using a clean glass serological pipette. Incubate for 20 min.
Repeat 3x for a total of 4 isopropanol washes. Carefully remove grids from isopropanol and air dry on a clean coverslip using tweezers.
NOTE: Residual isopropanol can make it difficult to release grids from tweezers. Making contact between the grid and the clean coverslip prior to releasing the grid from the tweezers can alleviate this problem.
Evaporate residual organics by transferring the coverslip with graphene-coated grids onto a hotplate set to 100 °C for 10 min. Cool to room temperature and store under vacuum until use.

10. UV/ozone treatment of graphene-coated grids

Using reverse-action tweezers, gently place grids into a clean illumination area of a UV/ozone cleaner with the graphene-coated side facing up.
Slide the illumination area closed and turn the machine ON. Turn the time dial to the designated treatment time to begin UV/ozone treating the grids..
NOTE: Treatment duration is a tunable parameter, with excess treatment having the potential to damage the grid. Han et al.³⁰ recommends 10 min of UV/ozone treatment, and we have also found a 10 min treatment to suffice. See step 12 for guidance on tuning this treatment time.
Proceed directly to plunging a cryoEM sample on the treated grids by following plunging steps as described in Koh et al.³⁷.
NOTE: Atmospheric hydrocarbons can accumulate on the grid surface after UV/ozone treatment and increase the hydrophobicity of the graphene surface³⁸^,³⁹. To avoid this, plunge the UV/ozone treated grids immediately. It may be advantageous to UV/ozone treat grids in batches, for example, UV/ozone treating six grids and immediately plunging those six grids, then UV/ozone treating a second batch of grids, followed by plunging the second batch.

11. Capturing a diffraction image

Ensure that the microscope is well tuned with parallel illumination established and set final defocus to -0.2 µm.
Insert the fluorescent screen and the beam stop fully. Enter diffraction mode to clearly visualize diffraction spots.
Acquire an image using a CCD camera and evaluate it using image analysis software.
NOTE: The most easily observed and identifiable diffraction spots generated by a graphene monolayer are 6 spots corresponding to a spatial frequency of 2.13 Å. Use a measurement tool to estimate the distance from the center of the diffracted beam to one of these diffraction spots in reciprocal units. Notably 2.13 Å 0.47 Å^-1.

12. Assessing grid hydrophilicity

Prepare the imaging setup. Locate a phone stand, tabletop imaging surface, phone with camera, glass coverslip, and paraffin film. Images shown here were obtained using a phone stand and tabletop imaging surface that were 3D printed using the provided .stl files, resulting in more easily reproducible and quantifiable contact angle measurements (Supplementary Figure 1A).
Lay a glass coverslip onto the flat surface, then cut a 1 cm by 1 cm square of paraffin film and place it onto the glass coverslip. Place phone on the phone stand, orienting the phone such that the camera is in-plane with the glass coverslip. Secure phone in this position using rubber bands (Supplementary Figure 1B). Take a sample photo to verify that the camera is aligned with the imaging surface.
Directly after UV/ozone treatment of graphene-coated grids, place a single grid onto the square of paraffin film on the glass coverslip. Ensure the graphene side is facing upwards. Add a 2 µL water droplet onto the center of the grid surface with a pipette and immediately take a photo.
NOTE: Repeat this step after: i) desired intervals of UV/ozone treatment to determine a sufficient length of treatment; or ii) desired time intervals post UV/ozone treatment to measure how long after treatment the grid surface maintains its hydrophilic character.
Calculate contact angles from photos by importing them into ImageJ⁴³ and using the contact angle plugin.

13. Single particle analysis of the dCas9 complex dataset

NOTE: All image processing described in this protocol was performed using cryoSPARC version 4.2.1.

Preprocess movies using Patch Motion Correction and Patch CTF Estimation jobs. Perform particle picking using the Blob picker job, using a spherical blob ranging in diameter from 115 Å to 135 Å.
Extract particles using the Extract from micrographs job, using normalized correlation coefficient (NCC) and power thresholds resulting in approximately 200-300 particles per micrograph. Note that appropriate thresholds and resulting particle count may vary, and users should inspect pick quality across a range of micrographs to identify suitable conditions.
Perform multiclass initial reconstructions using the Ab-initio reconstruction job, requiring three classes. Two of the three classes will likely contain non-Cas9 particles, including surface contaminants. Select the class resembling dCas9 for further processing.
NOTE: Additional rounds of multiclass Ab-initio reconstruction or Heterogeneous refinement may be applied to further refine the particle stack.
Perform 3D refinement using the Non-uniform Refinement job selecting default parameters. Estimate resolution of the reconstruction using the Validation (FSC), and ThreeDFSC jobs, employing the maps and mask from the final 3D refinement.

Representative Results

Successful fabrication of graphene-coated cryoEM grids using the equipment (Figure 1) and protocol (Figure 2) outlined here will result in a monolayer of graphene covering the foil holes that can be confirmed by its characteristic diffraction pattern. To promote protein adsorption to the graphene surface, UV/ozone treatment can be used to render the surface hydrophilic by installing oxygen-containing functional groups. However, hydrocarbon contaminants in the air can adsorb onto the graphene surface as early as 5 min post UV/ozone treatment and counteract this effect³⁸^,³⁹. Importantly, both the duration of UV/ozone treatment and the time elapsed between treatment and plunging can affect sample quality. We demonstrate these effects using a simple method for assessing the hydrophilic character of the coated grid based on the surface contact angle (Figure 3; see step 12).

To demonstrate the use of graphene supports in single particle cryoEM, we applied a catalytically inactive RNA-guided DNA endonuclease S. pyogenes Cas9 (H10A; C80S; C574S; H840A)⁴⁰ in complex with sgRNA and target DNA to graphene-coated grids, collected a cryoEM dataset from these grids, and performed single particle analysis⁷. Graphene-coated grids consistently contained ~300 particles per micrograph at 0.654 Å/pix magnification using a 300 keV microscope equipped with a K3 direct electron detector (Figure 4A-E). An 8 h data collection session with a +18° stage tilt yielded 2,963 movies and 324,439 particles in a final curated stack. Using these particles, we generated a 3D reconstruction which, upon refinement, yielded a density map with an estimated resolution of 2.7 Å and adequate angular sampling to avoid anisotropic artifacts (Figure 5). An atomic model (PDB 6o0z)⁴¹ was docked into this map, and refined using ISOLDE⁴². Residues R63-L82 of this fitted atomic model are displayed with the refined cryoEM density map, highlighting the resolved side-chain density (Figure 5B). When comparing the same sample and concentration (250 ng/µL) applied to identical grids that lacked graphene, no particles were observed (Figure 4F,G). This observation highlights the efficacy of the graphene support in enabling the visualization of particles from low-concentration samples.

Figure 1: Required equipment. Lab equipment and tools necessary for the fabrication of graphene grids using the protocol detailed in this article. Items and their quantity are shown and labeled accordingly. Requisite reagents that are not shown include: CVD graphene, methyl-methacrylate EL-6 (MMA), ammonium persulfate (APS), acetone, isopropanol, ethanol, molecular grade water. Requisite instruments that are not shown include: spin coater, glow discharger, hot plate, vacuum desiccator, and thermometer. All requisite items are detailed in the Table of Materials. Please click here to view a larger version of this figure.

Figure 2: Schematic of the graphene grid fabrication process. Graphene is coated with a thin layer of methyl-methacrylate EL-6 (MMA) using a spin coater (step 2). Graphene on the opposite side of the copper foil is removed via plasma etching (step 3). Ammonium persulfate (APS) is then used to etch away the copper (steps 4-5). The MMA-graphene film is placed onto the grid surface (step 6-7). Lastly, MMA is dissolved during a series of washes with organic solvents (steps 8-9). Steps indicated above arrows correspond to numbered steps described in the protocols section. This method has been adapted from Han et al.³⁰. Please click here to view a larger version of this figure.

Figure 3: Assessment of grid surface hydrophilicity as a function of duration of UV/ozone treatment and time elapsed post-treatment. (A) Measured contact angles plotted as a function of the duration of treatment. Decreased contact angles are consistent with increased hydrophilicity (untreated grid: 78°; 20 min: 37°). Contact angles measured using ImageJ⁴³. (B) Measured contact angles plotted as a function of time, post treatment (0 min: 45°; 60 min: 74°). Grid measured in the post treatment time-course was UV/ozone treated for 12 min, as indicated by asterisk. Each post-treatment measurement was performed on the same grid, with the sample removed by wicking between measurements. Specific contact angles measured are expected to vary as a function of laboratory environmental conditions, and we recommend that users perform similar experiments in their laboratories to identify suitable conditions. Please click here to view a larger version of this figure.

Figure 4: Representative images of graphene-coated grid and uncoated control grids. (A-C) Representative atlas, grid square, and foil hole images of graphene-coated holey carbon grids taken on a 300 keV microscope equipped with a K3 direct electron detector. (D) CryoEM micrograph of S. pyogenes dCas9 in complex with sgRNA and target DNA (complex at 250 ng/µL concentration) on graphene-coated holey carbon grid. (E) Diffraction image from grid imaged in panels (A-D). Orange arrow indicates a position corresponding to a spatial frequency of 2.13 Å. An identical sample to that in panel (D) was applied to (F) UV/ozone treatment and (G) glow discharged holey carbon grids without graphene. CryoEM micrographs displayed are representative of each grid and show no particles. Please click here to view a larger version of this figure.

Figure 5: CryoEM reconstruction of a Cas9 complex from graphene-coated grids. (A) CryoEM density map from 3D reconstruction of the S. pyogenes dCas9 in complex with sgRNA and target DNA. (B) Residues R63-L82 from a fitted model are depicted within the semi-transparent cryoEM density, with a subset of visible sidechains labeled. (C) Fourier Shell Correlation (FSC) curves of the unmasked, loosely, and tightly masked maps. (D) Histogram and directional FSC plot based on the 3DFSC method²³. See Supplementary Figure 2 and step 13 for more information. Please click here to view a larger version of this figure.

Supplementary Figure 1: Contact angle imaging stand. (A) A 3D printed camera stand and tabletop imaging mount to secure camera in a position that aligns the camera in-plane with the coverslip. (B) Grid is placed on the coverslip on top of a 1 cm x 1 cm square piece of paraffin film. The depicted imaging mount was 3D-printed using the provided .stl files (Supplementary Coding File 1 and Supplementary Coding File 2) and can be readily modified to accommodate most devices. Please click here to download this File.

Supplementary Figure 2: Image processing workflow. Processing workflow for dCas9 complex. Job names, job details, and non-default parameters (italicized) are indicated. Please click here to download this File.

Supplementary Coding File 1: Stereolithography CAD files in the STL format are provided to facilitate 3D printing of camera stand (camera_stand_v1.stl). Please click here to download this File.

Supplementary Coding File 2: Stereolithography CAD files in the STL format are provided to facilitate 3D printing of the tabletop imaging mount (slide_mount_v1.stl) and Please click here to download this File.

Discussion

CryoEM sample preparation involves a host of technical challenges, with most workflows requiring researchers to manually manipulate fragile grids with extreme care to avoid damaging them. Additionally, the amenability of any sample to vitrification is unpredictable; particles often interact with the air-water-interface or with the solid support foil overlaying the grids, which can lead to particles adopting preferred orientations or failing to enter the imaging holes unless very high protein concentrations are applied²⁴. Overlaying holey cryoEM grids with a continuous monolayer of graphene has shown tremendous promise in improving particle distributions on micrographs, increasing particle numbers at low concentrations, and reducing preferred orientations driven by interactions at the air-water-interface³⁰.

A limitation of existing graphene coating protocols for cryoEM grids is the extensive manual manipulations required for the coating process, which can compromise quality and increase grid-to-grid variability. In this work we describe slight modifications to an existing protocol for coating cryoEM grids with a monolayer of graphene³⁰.

Critical steps within this protocol include coating CVD graphene with MMA, dissolution of the CVD graphene copper substrate in APS, and the application of graphene to cryoEM grids. We modified the original protocol to minimize manual manipulations of the coated grids by exchanging solvents within the same Petri dish, instead of individually handling and transferring grids into a new solvent container for each wash step, thereby aiming to increase the yield of intact, high quality, graphene-coated grids. While we strove to reduce manipulations of graphene coated grids to a minimum, we acknowledge that the manual application of individual graphene squares to cryoEM grids is inherently challenging, and that some grid-to-grid variability is expected.

Graphene-coated grids typically require UV/ozone treatment to render the surface hydrophilic for sample application. The duration of UV/ozone treatment and the time elapsed after treatment and prior to plunging can impact grid hydrophilicity and ultimately sample quality. In addition to the grid fabrication protocol, we describe a technique for assessing grid hydrophilicity following UV/ozone treatment. In the procedure, the surface contact angle of an applied sample is used as an indicator of the hydrophilic character of the coated grid²⁰^,⁴⁴. Designs are provided to inexpensively 3D-print a custom grid imaging mount that utilizes a simple cell phone camera to estimate the surface contact angle.

Finally, we describe results obtained by employing this protocol to determine the 2.7 Å cryoEM structure of the catalytically inactive RNA-guided DNA endonuclease, S. pyogenes Cas9 in complex with sgRNA and target DNA⁴⁰. In the absence of graphene, no particles were observed in foil holes at the complex concentrations used (250 ng/µL). In contrast, graphene-coated grids bore particles at high density, enabling facile 3D-reconstruction of a high-resolution map from 2,961 micrographs. Taken together, these data highlight the value of applying graphene monolayers to cryoEM grids for single particle analysis.

Declarações

The authors have nothing to disclose.

Acknowledgements

Specimens were prepared and imaged at the CryoEM Facility in MIT.nano on microscopes acquired thanks to the Arnold and Mabel Beckman Foundation. Contact angle imaging devices were printed at the MIT Metropolis Maker Space. We thank the laboratories of Nieng Yan and Yimo Han, and staff at MIT.nano for their support throughout the adoption of this method. In particular, we extend our thanks to Drs. Guanhui Gao and Sarah Sterling for their insightful discussions and feedback. This work was supported by NIH grants R01-GM144542, 5T32-GM007287, and NSF-CAREER grant 2046778. Research in the Davis lab is supported by the Alfred P. Sloan Foundation, the James H. Ferry Fund, the MIT J-Clinic, and the Whitehead Family.

Materials

250 mL beaker (3x)	Fisher	02-555-25B
50 mL beaker (2x)	Corning	1000-50
Acetone	Fisher	A949-4
Aluminum foil	Fisher	15-078-292
Ammonium persulfate	Fisher	(I17874
Coverslips 50 mm x 24 mm	Mattek	PCS-1.5-5024
CVD graphene	Graphene Supermarket	CVD-Cu-2×2
easiGlow discharger	Ted-Pella	91000S
Ethanol	Millipore-Sigma	1.11727
Flat-tip tweezers	Fisher	50-239-60
Glass cutter	Grainger	21UE26
Glass petri plate and cover	VWR	75845-544
Glass serological pipette	Fisher	13-676-34D
Grid Storage Case	EMS	71146-02
Hot plate	Fisher	07-770-108
Isopropanol	Sigma	W292907
Kimwipe	Fisher	06-666
Lab scissors	Fisher	13-806-2
Methyl-Methacrylate EL-6	Kayaku	MMA M310006 0500L1GL
Molecular grade water	Corning	46-000-CM
Negative action tweezers (2x)	Fisher	50-242-78
P20 pipette	Rainin	17014392
P200 pipette	Rainin	17008652
Parafilm	Fisher	13-374-12
Pipette tips	Rainin	30389291
Quantifoil grids with holey carbon	EMS	Q2100CR1
Spin coater	SetCas	KW-4A	with chuck SCA-19-23
Straightedge	ULINE	H-6560
Thermometer	Grainger	3LRD1
UV/Ozone cleaner	BioForce	SKU: PC440
Vacuum desiccator	Thomas Scientific	1159X11
Whatman paper	VWR	28297-216

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Grassetti, A. V., May, M. B., Davis, J. H. Application of Monolayer Graphene to Cryo-Electron Microscopy Grids for High-resolution Structure Determination. J. Vis. Exp. (201), e66023, doi:10.3791/66023 (2023).