The present protocol describes that bipolar radiofrequency device-assisted laparoscopic partial splenectomy (LPS) is safe and effective. A bipolar radiofrequency device in LPS can reduce intraoperative bleeding and achieve the clinical effect of a “bloodless spleen incision”, which is worthy of clinical application.
Among the lymphatic system in the human body, the spleen is the most extensive one and has hematopoietic, hemofiltration, blood storage, and immune functions. As a new method of preserving the spleen, laparoscopic partial splenectomy (LPS) has been increasingly applied in clinical practice with people's deeper insights into minimally invasive treatment and the development of technical equipment. Compared with conventional open splenectomy, LPS can preserve normal spleen tissue as much as possible, decrease the occurrence of complications after total splenectomy, and reduce postoperative hospital stay. The bipolar radiofrequency excision hemostatic device used for LPS can solidify the splenic tissue and close the small blood vessels, which reduces the hemorrhage of the spleen cross-section and clears the operative field, thus achieving the ideal effect of "bloodless partial splenectomy". Therefore, under the premise of strictly mastering the indications and fully understanding the vascular anatomy of the spleen, the application of the bipolar radiofrequency excision hemostatic device in LPS is worthy of clinical promotion.
The spleen is the most extensive lymphatic system in the human body and has hematopoietic, hemofiltration, blood storage, and immune functions. Splenectomy is prone to complications such as thromboembolism, hemorrhagic, infectious, iatrogenic damage to adjacent organs, and overwhelming post-splenectomy infection (OPSI). Therefore, selective preservation of the spleen during the procedure has gradually attracted the attention of clinical surgeons1,2,3. Thus, sufficient splenic tissue must be preserved to maintain spleen function while ensuring complete resection of benign splenic tumors without recurrence. With the development of laparoscopic technology, laparoscopic partial splenectomy (LPS) has been gradually promoted in clinical practice, and its safety and effectiveness have been widely recognized4,5. LPS was first reported in 1995 by Poulin et al.6. Compared to total splenectomy, LPS has the advantage of reducing the incidence of postoperative complications and the length of the hospital stay.
The spleen is rich in blood supply. How to control and reduce bleeding in the process of resection is an urgent problem, especially in LPS. Therefore, various hemostatic energy devices have emerged and are applied to LPS, such as ultrasonic knife7, bipolar radiofrequency device8,9, bipolar electrocoagulation10, argon ion coagulation knife11, super suction knife11, and vascular closure device12. The high-frequency alternating current generated by the radio frequency electrodes is transmitted to the surrounding tissues. Bipolar radiofrequency excision hemostatic device has been widely used in hepatectomy because of its exact radiofrequency hemostatic effect but is rarely used in partial splenectomy13. Here, a method of bloodless laparoscopic partial splenectomy assisted by bipolar radiofrequency excision hemostatic device is described. The use of bipolar radiofrequency electrode-assisted LPS can effectively reduce intraoperative bleeding and improve the safety and effectiveness of surgery. The main objective of this method is to achieve the effect of partial splenectomy without blood by using a bipolar radiofrequency electrode, improve the safety and effectiveness of the operation, and facilitate clinical promotion. This procedure can be applied in most benign splenic diseases requiring partial splenectomy.
A 19-year-old male patient with no specific medical history was admitted to the hospital with a 2-week history of an occupying splenic lesion on physical examination. Physical examination showed no significant abnormalities. Laboratory tests such as routine blood, coagulation, and liver function were normal. A color Doppler ultrasound of the abdomen revealed a solid area of approximately 5.6 x 5.1 cm2 between the spleen and kidney, which was thought to originate from the left adrenal gland. Enhancement magnetic resonance imaging (MRI) of the upper abdomen showed a 5.1 x 4.6 cm2 hyperintense round occupying the upper pole of the spleen (Figure 1). The admission diagnosis revealed an occupying lesion in the spleen, likely a hemangioma. Upon admission, a preoperative evaluation was conducted. The patient had no contraindications to surgery. LPS was performed because of the patient's age, the many possible complications after total splenectomy, and the willingness of his family to undergo partial splenectomy.
The present human surgery protocol is approved by and follows the ethical guidelines of the Zhujiang Hospital, Southern Medical University (Guangzhou, China). Informed consent was obtained from the patient for the release of information and data related to his treatment.
1. Preoperative preparation
2. Surgical technique
3. Postoperative care
The patient was doing well after the surgery and was sent back to the ward. The operation lasted 120 min, with intraoperative blood loss of about 100 mL and about 2,600 mL of fluid replacement, without blood transfusion. Intraoperative urinary output was 600 mL. The patient recovered well with no postoperative complications and was discharged on the 6th postoperative day. Postoperative pathology showed a splenic capillary hemangioma with active cell growth and a size of 4.7 x 4.0 x 3.5 cm3. The spleen was dissected with an ultrasonic knife along the coagulation of the necrotic area, and no significant bleeding during dissection of the spleen. The spleen section showed coagulated necrotic tissue without obvious active hemorrhage (Figure 4, Table 1).
Figure 1: Enhancing magnetic resonance imaging confirmed a hyperintense round occupying the upper pole of the spleen. (A) Cross-sectional imaging. (B) Coronal view imaging. Please click here to view a larger version of this figure.
Figure 2: Vascular branches of the spleen. The figure shows (a) the splenic artery, (b) the superior pole branch of the splenic artery, (c) the superior pole branch of the splenic vein, and (d) the tumor. Please click here to view a larger version of this figure.
Figure 3: Ischemic line in the upper pole of the spleen. To block blood flow to the spleen's superior pole after clamping the splenic artery's superior pole branch, the ischemic line was observed. Please click here to view a larger version of this figure.
Figure 4: Bipolar radiofrequency ablation of the spleen. To lift the upper pole of the spleen with forceps, a bipolar radiofrequency hemostatic device was used along the ischemic line on the surface of the spleen to perform coagulation and ablation. Please click here to view a larger version of this figure.
Figure 5: The residual spleen section after partial splenectomy. Please click here to view a larger version of this figure.
Items | Resultados |
OP time (min) | 120 |
Operation ABL (mL) | 100 |
Operation ABT (mL) | 0 |
Fluid replacement (mL) | 2600 |
Postop complications | None |
Discharge time | The 6th postoperative day |
Table 1: Relevant outcomes of LPS. LPS, laparoscopic partial splenectomy; OP, operation time; ABL, amount of blood loss; ABT, Amount of blood transfusion.
With the development of modern medicine, especially immunology, there is a better understanding of the anatomical structure and function of the spleen. In the past, many benign and malignant spleen diseases were treated by total splenectomy due to the limitations of knowledge and surgical techniques. In 1992, Delaitre et al. first reported laparoscopic splenectomy, and laparoscopic techniques began to be gradually applied to spleen surgery14. However, studies have shown complications such as thromboembolism, hemorrhage, infections, congenital damage to adjacent organs, and dangerous postoperative infections occur after total splenectomy2,3. Therefore, for patients with splenic diseases, the initial blind resection treatment has been gradually replaced by selective splenic preservation therapy, such as splenic repair suture, partial splenectomy, splenic artery embolization, and splenic transplantation. In 1995, Poulin et al. first reported LPS, which opened a new chapter in splenic surgery6. With a more comprehensive understanding of the function of the spleen, the anatomical relationship between the splenic lobules and segmental blood supply, and the application of various energy devices, LPS has been applied to patients in major centers15,16,17,18. LPS can preserve as much normal tissue of the spleen as possible while removing the diseased tissue, reducing various complications after total splenectomy. Compared with open partial splenectomy (OPS), LPS requires a longer time and more intraoperative bleeding. However, it does not increase the incidence of postoperative complications and delay postoperative recovery. On the contrary, studies have shown that LPS has a significantly lower postoperative complication rate and a significantly shorter postoperative hospital stay than OPS18,19,20.
The blood supply to each segment of the spleen is the anatomical basis of LPS. There are mainly two-lobe and four-segment types, i.e., the upper and lower segments of the spleen, the middle and upper segments of the spleen, the middle and lower segments of the spleen. It can also be divided into the splenic hilar, intermediate, and peripheral areas. The splenic artery divides into splenic lobular vessels at the splenic hilum, including 1, 2, 3, and multivessel types. The most common type is the 2- and 3-vessel type, in which the trunk of the splenic artery branches off 2 or 3 vessels of the splenic lobe and enters the spleen. In this regard, there are few arteriovenous anastomoses between adjacent splenic lobes (segments), forming an irregular plane with almost no vascular zone. This anatomical feature justifies the feasibility of partial splenic resection to a certain extent. Partial splenic separation can be performed in a relatively avascular zone to reduce the amount and rate of bleeding21,22. Surgeons can choose different types of partial spleen resections depending on specific conditions and the anatomy of the splenic blood supply. In this case, the tumor was in the spleen's upper part. After releasing and ligating the branch vessels in the upper part of the spleen, a clear ischemic line appeared on the surface of the spleen, based on where partial splenectomy is performed.
The spleen is rich in blood supply, and many blood vessels in the spleen parenchyma need to be dealt with during resection, which results in long operation time, heavy bleeding, and high risk. Therefore, various hemostatic energy devices have emerged and are applied to LPS7,8,9,10,11,12,23,24,25. A bipolar radiofrequency device is a bipolar radiofrequency electrode containing two pairs of 5 cm long reversed electrodes displayed in a rectangular array. The high-frequency alternating current generated by the radio frequency electrodes is transmitted to the surrounding tissues. After the alternating current passes through the tissue, molecules in the tissue rub against each other, generating heat along the current direction, resulting in ischemic necrosis of cells and forming a coagulated necrotic zone approximately 1 cm in width. In 2008, Professor Habib invented the Habib 4X bipolar radiofrequency cutting hemostatic electrode and used it in liver resection with promising results26. Subsequently, the bipolar radiofrequency device was gradually promoted for hepatectomy in major centers. Wang et al. used a bipolar radiofrequency device for LPS for the first time in China and achieved the therapeutic result of bloodless splenectomy27.
This patient underwent successful LPS using a bipolar radiofrequency device. Our experience is summarized as follows: (1) Strict indications, including trauma to the spleen, benign spleen tumors, splenic cysts, hematomas, and especially hematologic disorders of the splenic margins, required splenectomy. Partial splenectomy was contraindicated for tumors near the splenic gallbladder. In addition, some studies have shown that at least 25% to 30% of the residual spleen needs to be preserved to maintain normal spleen function17,18. (2) Preoperative CT and intraoperative ultrasonography were used to clarify the anatomical relationship between the lesion, the splenic artery, and its branches. After dissecting the splenic artery trunk, an disposable single-cavity rubber catheter was placed as a pre-blocking band to block the splenic pedicle and reduce bleeding on time when there was massive bleeding during surgery. The splenic pedicle could be fully exposed by pulling the catheter to protect important vessels and expose the bleeding site. (3) The splenic artery was dissected along the main splenic artery toward the splenic hilum to avoid injury to the pancreas. Attention was paid to identifying the direction of the branch vessels of the secondary splenic pedicle. The vessels in the splenic lobe to be resected were accurately clamped. The ischemic borders of the spleen were observed. Because some patients have anatomic variations in the branches of the splenic artery, careful identification of the anatomical relationship was required, and the vessels need to be carefully released. Only when the ischemia line has been found, the vessels must be severed. To ensure the viability of the spleen, a partial splenectomy could be performed after confirming the resection surface at approximately 1 cm on the blood supply side of the ischemic line. (4) Intraoperative operations were handled elaborately with moderate freedom, and the collateral blood vessels of the spleen were preserved to protect the secondary blood supply system of the spleen. When preserving the upper pole of the spleen, the upper part of the spleen and the gastric ligament must not be cut to avoid damaging the short gastric vessels and the blood supply to the upper pole of the spleen. When preserving the lower pole of the spleen, the lower part of the gastrosplenic ligament (splenic colonic ligament) needs to be protected to avoid damaging the left gastrointestinal vessels and the blood supply to the lower pole of the spleen. (5) The appropriate power for the bipolar radiofrequency device must be selected, and deep ablation and coagulation must be performed. After ablation disconnection, the broken end of the vessel was clamped using non-absorbable polymer locking clips, or a thicker tube was ligated and sutured to the splenic portion to merge the seal. This patient underwent the procedure successfully with no complications, validating the safety and feasibility of bipolar radiofrequency device-assisted LPS, but further exploration and validation in multicenter, large sample size is still needed.
This surgical method mainly applies to young patients with benign splenic tumors. It is not suitable for large benign tumors of the spleen, malignant tumors of the spleen, and the following situations: the residual spleen is too small to lose function after partial resection, and the branches of the spleen vessel are difficult to be exposed because of serious adhesion or vascular anomalies.
In conclusion, bipolar radiofrequency device-assisted LPS is safe and effective. Under the premise of strictly mastering the indications and fully understanding the vascular anatomy of the spleen, the application of a bipolar radiofrequency device in LPS can reduce intraoperative bleeding and achieve the clinical effect of "bloodless spleen incision", which is worthy of clinical application.
The authors have nothing to disclose.
This research was supported by the National Natural Science Foundation of China (Grant No. 82072627).
Absorbable hemostat | Ethicon, LLC | W1913T | |
Absorbable medical film | Shanghai Divine Medical Technology Co., Ltd | 60007 | |
Bipolar radiofrequency excision hemostatic device | Angio Dynamics, Inc | Rita1500X | Tools for spleen resection and coagulation of small vessels |
pneumoperitoneum needle | Unimicro Medical Systems Co.,Ltd | 150mm | |
Disposable spiral negative pressure drainage pipeline | Jiangsu Aiyuan Medical Technology Corp | 424280 | Drainage of abdominal residual fluid |
Disposable trocar | Kangji Medical | 10004, 10006 | |
Laparoscopic system | Olympus | WM-NP2 L-RECORDOR-01 |
Laparoscopic camera system and supporting display screen |
Non-absorbable polymer ligation clips (Hem-o-lok) | Teleflex Medical | 544230 | |
Ultrasound knife | Johnson | GEN11 | Tools for spleen resection |
Vicryl rapide | Ethicon, LLC | 2-0, VCP345H 90010 | Suture incision and Trocar hole |
Disposable single-cavity rubber catheter | Yangzhou Huayue Technology Development Co., Ltd | 3.5mm (10Fr) | |
Video system | Lenovo | GK309 | |
Electrocardiographic monitor | Philips Goldway (SHENZHEN) Industrial, Inc | UT4000B | Postoperative ecg monitoring |
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