记录神经反应和注射示踪剂一个清醒小鼠的制备
Summary
重要的是理解大脑功能和指导安置途径示踪染料神经反应的电特性。然而，许多研究都在麻醉动物。理解没有麻醉药的脑功能，我们开发了一种方法来记录神经元的反应特性，并在清醒小鼠注入染料。
Abstract
这是众所周知的麻醉，神经反应特性改变在大脑的不同区域。13。在听觉系统，脑干神经元，包括阈值，频率特异性，抑制边带根本性的响应特性，在麻醉下1-2显着的方式被改变。这些观察提示生理学家设法从单个神经元的记录，没有麻醉的污染影响。其结果之一是去大脑的准备，完全是在中脑4水平横断脑干。该制剂的弊端，是一项艰巨的手术，消除降的预测从脑，无法用感官刺激，检查以上的脑结构。不同的策略已植入电极阵列，长期记录单个神经元和多单元集群，而动物是清醒和/或行为5,6 </suP>。然而，这些技术与示踪染料注射后，电生理特征的大脑结构不兼容。为了避免改变与麻醉药的神经反应性能，同时记录单个神经元的电生理反应特性，我们已经适应了长期用鼠标10-12蝙蝠7-9头部约束技术。使用这种方法，我们能够在麻醉的小鼠进行了几天的电生理记录。我们可以在录音结束，然后注入一种染料，重建电极位置和记录点，或注入示踪剂，所以可确定，并从记录位点的途径。这种方法无需使用麻醉剂以及隔离多天的单神经元记录。
Protocol
Discussion
后头部约束系统的电生理及神经解剖实验小鼠的优势是可以进行麻醉的，清醒小鼠实验，消除潜在的响应由于麻醉药品的污染。此外，一套最多可以使用多天，以便更有效地使用动物。
头后的大部分组件是可用的现成的。只有头部后和安装设备的定制，这两者是相对简单的建设，是可以重复使用。如果一台机器的店是不是在一个机构，研究者可能使用<a href="http://www.emachineshop…
Declarações
The authors have nothing to disclose.
Acknowledgements
0920060 NSF资助，NIH资助DC004395 NHMRC赠款1009482，科学和医学研究的新南威尔士州办事处，加内特过时和罗德尼·威廉斯纪念基金会的支持。
Materials
| Name | Company | Catalogue number | Comments |
| Etch gel for cleaning skull prior to dental cement | Henry Schein | 101-5396 | 12/pk with 1.2 mL syringes |
| Primer for dental cement | Kerr Optibond | 25881 | 8 mL bottle |
| Adhesive for dental cement | Kerr Optibond | 25882 | 8 mL bottle |
| Applicators for dental cement | Kerr Optibond | 24680 | 200/pk |
| Dental Cement | Charisma, Heraeus Kulzer | 66000085 | 4gm Syringe Refill |
| Tungsten Rod (Ground Pin) | A-M Systems | 717200 | 0.010″ diameter |
| Economy UV Curing Light | Henry Schein | CU-80 | |
| Head-post | Built in-house | ||
| Mounting bar | Built in-house | ||
| Mounting block | Built in-house | ||
| Stereotaxic Frame | David Kopf Instruments | 902 | |
| Mouse and Neonatal Rat Adaptor | Stoelting | 51625 | |
| Deltaphase Isothermal Pad | Braintree Scientific, Inc. | 39DP |
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