Source: Zychlinsky Scharff, A., et al. Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice. J. Vis. Exp. (2017).
This video demonstrates the establishment of uropathogenic Escherichia coli infection in a female mouse bladder through transurethral catheter instillation. The injected bacteria successfully establish a urinary tract infection within the bladder, providing a valuable platform for studying healthcare-associated diseases related to the urinary tract.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Preparation of Catheters
2. Preparation of Uropathogenic E. coli for Infection
3. Preparation of Mice
4. Transurethral Instillation of Male Mice
Figure 1: The Quality of Instillation does not Correlate with Bacterial Burden at 24 hr Post-infection. Six to 8 week-old female and male C57Bl/6 mice were instilled with 107 CFU of UPEC. Immediately after each instillation, a single researcher assigned a quality score to the instillation, as defined by specific criteria (boxed text). 24 hr post-infection, bladders were aseptically removed to enumerate bacterial burden. The plot depicts the assigned quality score versus CFU/bladder. Each dot is one mouse, and 5 pooled experiments are shown. p = 0.17, Kruskal-Wallis test comparing the mean rank of each column with the mean rank of every other column with post hoc Dunn's test for multiple comparisons.
The authors have nothing to disclose.
BD Insyte Autoguard Shielded IV Catheters 24 G, 0.7 mm external diameter, 14 mM long | BD Medical | 381811 or 381411* | * catalog number is country dependent |
Inoculating loop | Greiner Bio-One | 731171 | |
syringes | B Braun | 9166017V | |
Thumb (Adson) forceps | Fine Science Tools | 11006-12 | |
insulin syringe | Terumo | BS05M2913 | |
5 ml tubes, polypropylene | Falcon | 352063 | |
LB Miller broth | Difco | 244620 | |
LB Miller agar | Difco | 244520 | |
Cuvettes | Bio-rad | 223-9955 |