Hematoxylin and Eosin Staining to Assess Colon Damage in DSS-Induced Colitis
Published: August 31, 2023
Abstract
Source: Du, Y., et al. Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model. J. Vis. Exp. (2018)
This video demonstrates the hematoxylin and eosin (H&E) staining method to evaluate colon damage in a murine model of DSS-induced colitis — an inflammatory bowel disease. Upon isolating the colon from a DSS-treated mouse and obtaining sections of the tissue, H&E staining of the sections helps identify the inflammation-mediated tissue damage.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Mice and DSS Keep the knockout (α-gustducin-/-) mice and age-, gender-, and body weight-matched wild-type control (α-gustducin+/+) C57BL/6 mice individually in clean cages. NOTE: The knockout mice have been backcrossed with C57BL/6 mice for over 20 g…
Declarações
The authors have nothing to disclose.
Materials
| Dextran Sulfate Sodium Salt (DSS) | MP Biomedicals | 2160110 | |
| H&E Staining Kit | BBI Life Sciences | E607318 | |
| Phosphate-Buffered Saline (PBS) | Sangon Biotech | B548117 | |
| BD 10 ml Syringe | BD Biosciences | 309604 | |
| Instruments and equipment | |||
| Balance | |||
| Scissors | |||
| Forceps | |||
| Staining jars | |||
| Software | |||
| Imag-Pro Plus | Media Cybernetics, Inc. |
Tags
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Hematoxylin and Eosin Staining to Assess Colon Damage in DSS-Induced Colitis. J. Vis. Exp. (Pending Publication), e21615, doi: (2023).